R/plate_functions.R
create_colkey_4diln_2ctrl_in_24.RdCreates a 24-column key for primer calibration, with 2x biol_reps and 2x tech_reps, and 5-fold dilution until 5^4 of +RT; then -RT (no reverse transcriptase), NT (no template) negative controls. That is a total of 6 versions of each sample replicate.
create_colkey_4diln_2ctrl_in_24(
dilution = c(5^(0:-3), 1, 1),
dilution_nice = c("1x", "5x", "25x", "125x", "-RT", "NT"),
prep_type = c(rep("+RT", 4), "-RT", "NT"),
biol_rep = rep(c("A", "B"), each = 12, length.out = 24),
tech_rep = rep(1:2, each = 6, length.out = 24)
)| dilution | Numeric vector of length 6 describing sample dilutions |
|---|---|
| dilution_nice | Character vector of length 6 with nice labels for sample dilutions |
| prep_type | Character vector of length 6 describing type of sample (+RT, -RT, NT) |
| biol_rep | Character vector of length 6 describing biological replicates |
| tech_rep | Character vector of length 6 describing technical replicates |
tibble (data frame) with 24 rows, and columns well_col, dilution, dilution_nice, prep_type, biol_rep, tech_rep.
Other plate creation functions:
create_blank_plate(),
create_colkey_6_in_24(),
create_colkey_6diln_2ctrl_in_24(),
create_rowkey_4_in_16(),
create_rowkey_8_in_16_plain(),
display_plate(),
label_plate_rowcol()
create_colkey_4diln_2ctrl_in_24()
#> # A tibble: 24 x 6
#> well_col dilution dilution_nice prep_type biol_rep tech_rep
#> <fct> <dbl> <chr> <fct> <fct> <fct>
#> 1 1 1 1x +RT A 1
#> 2 2 0.2 5x +RT A 1
#> 3 3 0.04 25x +RT A 1
#> 4 4 0.008 125x +RT A 1
#> 5 5 1 -RT -RT A 1
#> 6 6 1 NT NT A 1
#> 7 7 1 1x +RT A 2
#> 8 8 0.2 5x +RT A 2
#> 9 9 0.04 25x +RT A 2
#> 10 10 0.008 125x +RT A 2
#> # … with 14 more rows