Remove baseline from qPCR amplification curves by subtracting median of initial cycles.
debaseline(plateamp, maxcycle = 10)| plateamp | data frame with plate amplification data, including variables well, cycle, fluor_raw (raw fluorescence value), and program_no. Assume program 2 for amplification curves from Roche LightCycler format data. |
|---|---|
| maxcycle | maximum cycle value to use for baseline, before amplification. |
platemap with additional columns per well:
| fluor_base | baseline /background value |
| fluor_signal | normalized fluorescence signal, i.e. fluor_raw - fluor_base |
BETA function version because:
- assumes Roche LightCycler format, we should ideally replace "program_no == 2" by something more generic?
- the rule-of thumb "baseline is median of initial 10 cycles" has not been tested robustly