Calculate dR/dT of melt curves for of every well in a plate.

dR/dT, the derivative of the melt curve (of fluorescence signal R vs temperature T), has a maximum at the melting temperature Tm. A single peak in this suggests a single-length PCR product is present in the well.

calculate_drdt_plate(platemelt, method = "spline", ...)

getdRdTall(platemelt, method = "spline")

Arguments

platemelt	data frame describing melt curves, including variables well, temperature, fluor_raw (raw fluorescence value).
method	to use for smoothing: "spline" default, uses smoothing spline stats::smooth.spline. "diff" base::diff for lagged difference
...	other arguments to pass to smoothing method.

Value

platemelt with additional column dRdT.

Functions

• getdRdTall:

See also

Other melt_curve_functions: calculate_dydx_1()